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We were particularly interested in the behaviour of two sub-clusters respectively containing mRNAs coding for keratins and keratin-associated proteins (Fig. 1A, black arrows), and S100 calcium binding proteins (Fig. 1A, red arrows).
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Cluster I consists of two sub-clusters, where the first sub-cluster, Ladakh-Sikkim sub-cluster, includes 4 populations from Ladakh [Ladakh Buddhist, Argon, Drokpa and Balti] and 2 populations from Sikkim [Bhutia and Lepcha].
In addition to these three clusters, a fourth distinct cluster, consisting of two sub-clusters, was observed, at the junction of the O. glaberrima-indica-japonica axes.
CL3 is composed of two sub-clusters.
Conversely, three out of four sub-clusters were significantly enriched in functional categories for the EO dataset (Fig. 7b).
The transitions between the 7- cluster and 4- cluster solutions include the merger of two sub clusters into Cluster 1 and the merger of three sub clusters into Cluster 3.
The composition of these two sub-clusters is summarized in supplemental Table S6.
Secondly, two sub-clusters of nDNA-encoded transcripts with significantly different expression patterns were observed.
The HCL tree was split into six clusters and two of these clusters further divided two sub-clusters.
While BO and LO were almost entirely composed of sub-cluster A and B, these two sub-clusters made up at least 55% of all other countries (except for GE which was devoid of sub-cluster A; Figure 5B).
Note that the splitting of one 100% pure cluster into two sub-clusters will let the BHI value unchanged.
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