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We used time-lapse microscopy to visualise the behaviour of embryonic macrophages in response to wounding and stimulation with a microbial-derived particulate antigen.
LIII hemocytes are sessile and quiescent but they are activated towards an exploratory behaviour at WPP, similar to the behaviour of embryonic hemocytes (Evans et al., 2010; Sampson and Williams, 2012a; Sampson and Williams, 2012b).
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Yet scientists risk their federal grants if they try to derive embryonic stem cells using alternative methods.Another challenge has to do with the long-term behaviour of human embryonic stem cells no matter their provenance both in the test tube and in the human body.
We also analysed the short-term behaviour of the embryonic Crx-expressing donors soon after transplantation into the adult retina by analysing retinal sections at 4 and 10 days post-transplantation. Specifically, we assessed whether integrating cells initially expressed the cone marker Rxrγ.
We focused, in particular, on the behaviour of the CrxGFP embryonic donor cells, as they demonstrated cone integration potential.
From these findings concerning the behaviour of MeCP2 during embryonic development, it also seems to be plausible that higher levels of MeCP2 mRNA can be found in better-differentiated human breast cells, indicated by OR positivity.
Given the assumption that the sorting observed in vivo is mechanistically analogous to the one characterized in vitro, one could predict that the behaviour of RoboSD in the embryonic CNS would not be dissimilar from the one just characterized in the COS cell assays.
Fibroblast growth factors (FGFs) constitute a family of structurally related proteins, which regulate many facets of cell behaviour, from embryonic patterning, to tissue repair and metabolism [ 1- 3].
To explore the specificity of the events we observe with the invasive breast cancer cells, we analysed the behaviour of non-tumourigenic 3 T3 mouse embryonic fibroblasts and weakly tumourigenic 293 T human embryonic kidney cells within the zebrafish embryonic model.
These interactions are key to the organizational behaviour of cell populations and contribute to the formation of embryonic tissues and the function of normal tissue in the adult organism.
Hypoxia usually correlates with malignant behaviours in cancer cells, but it has been recently involved, together with Wnt signalling, in the differentiation of embryonic and neural stem cells.
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