Abercrombie, M. & Heaysman, J. E. Observations on the social behaviour of cells in tissue culture.
However, the 2D cell model might not faithfully capture the physiological behaviour of cells in vivo.
Recording intracellular (IC) bioelectrical signals is central to understanding the fundamental behaviour of cells and cell networks in, for example, neural and cardiac systems1,2,3,4.
Full-length and truncated domains of the three proteins were fused to green fluorescent protein (GFP), expressed in NIH3T3 cells, and distribution and behaviour of cells were analysed by using digitally enhanced differential interference contrast (DIC) and fluorescence video microscopy.
At the same time, coupling this capability to the mature field of DNA micropatterning affords additional opportunities, such as single-cell resolution and high-throughput screening to discover the behaviour of cells in their niche.
The behaviour of cells cultured within three-dimensional (3D) structures rather than onto two-dimensional (2D) culture plastic more closely reflects their in vivo responses.
Tissue engineering is a multidisciplinary area, which requires considerable biological input to gain insight into the behaviour of cells during in vitro culture and in vivo implantation.
Development of new materials for tissue engineering can be facilitated by the capacity to efficiently monitor in vivo the survival, proliferation and differentiation behaviour of cells implanted in different target tissues.
The association of inorganic phases (amorphous silica) to form chiral hybrid materials will also be described so as the behaviour of cells (fibroblast adhesion and migration) when seeded on these dense biomimetic matrices.
We propose that these robust and stable matrices can be conveniently and routinely used in the tissue culture laboratory to study the behaviour of cells grown in three-dimensions.
To describe the behaviour of cells adhering and proliferating over different types of (and/or differently treated) substrates, some mathematical models have been also suggested in literature; these models consider both the dependence of cell adhesion/proliferation over time, and the influence of substrate morphology in allowing (or even hampering) cell attachment.
