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The development of Parkinsonism was assessed by three conventional behavioral tests, compared to the effects of unilateral 6-hydroxydopamine (6-OHDA) — induced lesions in the nigrostriatal pathway.
Plasma testosterone levels were significantly lower in RbNO males compared to untreated males and did not increase during the behavioral tests compared to sham operated males.
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Nevertheless, treadmill training produced essentially no difference in behavioral deficits on most tests compared to untrained animals.
Animals receiving atipamezole plus rehabilitation exhibited significantly greater motor improvement in both behavioral tests as compared to vehicle-treated animals receiving rehabilitation.
Furthermore, neurotrophin-3 (NT-3) administration combined with in vivo transplantation, significantly increased the survival of grafted cells and improved the behavioral test results compared to the cell transplantation only group.
Firstly, we used the novel object recognition test and observed that these mice displayed completely normal behavioral performances in the 1 day retention tests as compared to their wild-type littermate control (Figure 1E).
Notably, MBL mice showed attenuated behavioral deficits when compared to their WT counterpart at 2 to 4 weeks postinjury (P < 0.01 for both Neuroscore and Beam Walk test).
The superior prediction power of MVPA compared to behavioral tests may justify the additional cost of MRI scans.
Morris water maze is a commonly used behavioral test to compare spatial learning and memory between various treatments and also assess spatial learning and memory as a function of the hippocampus [ 12, 18, 25, 26].
The data recorded from the behavioral tests were compared using Kruskal-Wallis ANOVA followed by Dunn's method for post hoc comparison.
In the present study, GPS at 100 200 mg/kg, but not 50 mg/kg, showed an equivalent efficacy on the anxiety behavioral tests, as compared with GP-EX at 50 mg/kg.
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