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Behavioral testing was used to assess neurological deficits.
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Behavioral tests were used to assess forelimb placement during locomotion, forelimb-use asymmetry, and forepaw tactile sensitivity.
Naïve mice (i.e., mice not exposed to any other behavioral test) were used for these physical examinations.
Behavioral tests were used to evaluate all the animals in this study.
For the behavioral analysis, Student's t-test was used to confirm the significance of differences between the means of groups.
One-way or two-way ANOVA, followed by the Tukey's HSD test, was used for behavioral analysis, and the nonparametric Kruskal Wallis test was used for the wire hanging tests.
The stay-on-hand test was used to quantify behavioral responses to forced stimulation by a human.
The Mann–Whitney U -test and Kruskal Wallis test followed by Dunn's multiple comparison test was used to analyze behavioral data.
The statistical analysis employed ANOVA for the body weight variation, body fat weight, and biochemical analyses, while the Kruskal-Wallis test was used for the behavioral analyses, with a 5%% level of significance.
Since the scores obtained for most dependent variables are not distributed normally, the Mann–Whitney U-test was used to compare migraine and tension-type headache in behavioral measures, and the Wilcoxon signed ranks test was used to compare repeated measures within the same sample.
For the ketamine behavioral results t-test were used to compare shams to controls.
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