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All behavioral testing was completed in a blinded manner.
After behavioral testing was completed mice, except those noted below, were prepared for histological verification of bulb-x.
After the behavioral testing was completed, all of the animals were deeply anesthetized with sodium pentobarbital (80 mg/kg, i.p).
The rats in the first trial received their oral treatment in the late afternoon after all behavioral testing was completed, whereas the rats in the second trial received their treatment in the early morning before any behavioral testing was performed.
After the behavioral testing was completed, the animals (n = 60) were deeply anesthetized with urethane 40% (1 g/kg, from Sigma Aldrich, France), and then transcardially perfused with saline (0.9%), followed by 4% paraformaldehyde in phosphate-buffered saline (0.2 M).
To confirm further the lack of connectivity between the patent nasal cavity and ablated bulb in a UNO mouse, HRP was injected in the still open naris of Mouse S (see Fig. 2) after its behavioral testing was completed [ 5, 9].
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The mice were group-housed, except when undergoing the behavioral tests, and kept with the same mice until all of the behavioral tests were completed.
At 13 weeks, when the behavioral tests were completed, the brain Hg concentration was comparable to the control (nonexposed) level (approximately 5 ng/g in both the exposed and control brains).
29 tests were completed successfully.
The taste behavioral testing was performed as previously described [16].
All behavioral testing was done on age-matched male mice.
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