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3) An alternative hypothesis for miR-124 behavioral phenotypes is that increased levels of tra in miR-124 mutants results in altered development/morphology of sex-specific neural circuitry.
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Our current hypothesis concerning the rotation-resistant behavioral phenotype is that resistant females show reduced fidelity to corn as a host (Levine et al. 2002; Spencer and Levine 2008).
Furthermore, a distinct characteristic of this RNAi behavioral phenotype is that the animals eventually display a combination of cilia- and musculature-driven locomotion (Fig. 6; supplementary material Movies 1 4).
Our finding of pervasive epistasis, at least among behavioral phenotypes, is in line with that recently described for other model organisms (e.g., Zwarts et al. 2011; Huang et al. 2012).
The genetic basis of these tame and aggressive behavioral phenotypes was confirmed in experiments that included cross-fostering of pups, transplantation of embryos and cross-breeding experiments [ 30, 25, 26, 32].
We observe that fru behavioral phenotypes are specified by either a single isoform or a combination of isoforms.
Further investigations using mice of different background strains and identification of biochemical pathways that elucidates the behavioral phenotypes are needed for better understanding of RAGE in basal states.
Although it is very possible that our behavioral phenotypes are a result of the loss of the putative deacetylase domain, they could also be due to the low expression of the N-terminal domain.
Interestingly, Figure 3B shows that all these behavioral phenotypes are restricted to the CCI models of neuropathic pain.
All behavioral phenotypes were analyzed with a factorial experimental design.
Although physical phenotypes like height and weight are easier to quantify, behavioral phenotypes are intensely studied in animals and humans.
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