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Behavioral assays were executed at variable phases of the circadian clock of the flies.
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DNase I and 'in gel' copper-phenantroline (Cu-OP) footprinting assays were executed as described [ 22, 29].
This is because cells can be incubated and observed within the same microenvironment in which the protein assays are executed.
All behavioral assays were performed on 100 mm chemotaxis media (CTX) petri plates5 at 20 °C.
Microspectrophotometry, electroretinogram recording and behavioral assays were used to determine visual pigments, retinal spectral sensitivity and whole-animal spectral responsivity, respectively.
Behavioral assays were performed as previously described [9], [13], [40].
Behavioral assays were performed on multiple cohorts of mice.
Behavioral assays were also performed to assess potential alterations in brain function.
All behavioral assays were conducted monthly following pellet implantation.
Behavioral assays were performed at 25° at 70% humidity.
All behavioral assays were conducted with experimenter blind to genotype of the worms assayed.
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