To further understand the role of RHO-1 signaling in adult behaviors it will be necessary to define the upstream activators and downstream effectors that modulate these behaviors.
In order to understand the specificity of the action of octopamine in so many different types of behaviors, it will be necessary to describe not only where octopamine is released but also the distribution of different octopamine receptor subtypes in the brain.
The investigation of effects of strain rate as well as tested temperature on the deformation behavior for it will be necessary.
Therefore, a more precise imitation of insect behavior during herbivory will be necessary, allowing imitation of both the pattern and amount of damage in a quantifiable and repeatable manner.
Further studies using a combination of genetics, behavior, and electrophysiology will be necessary to validate these candidates and to elucidate more precisely how they mediate the biological effects of TDP-43 expression or loss of function in motor neurons.
If we are to reach definitive proof of microbial endocrinology as a mechanism by which the microbiota can influence the brain, and ultimately behavior, then it will be necessary to identify those bacteria which interface with elements of the CNS and ENS and the mediators that they produce that enable such communication.
Both hypotheses allow us to explain the observed electrical behavior; nevertheless, further investigations will be necessary to clarify the involved physical mechanisms and to adjust the conception of the porous silicon-based AC switch periphery.
However, further experimental studies of allosteric proteins, including techniques that probe the dynamic behavior of aromatic side chains, will be necessary to comprehensively understand the structural prerequisites for dynamic allosteric coupling.
Other interventions will be necessary, too, including behavior change, syringe exchange, and structural approaches.
However, further studies will be necessary to clarify the actual difference in behavior between the CSCs and non-CSCs during the processes required for metastasis in human cancer.
