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To evaluate whether the Ligia sequences examined exhibit clocklike behavior, we used a Likelihood Ratio Test on the likelihood scores obtained with PAUP* [v. 4b10, 41] of the tree in Figure 3 with and without molecular clock constraints.
To study how mural cells affect endothelial cell behavior we used a genetically and pharmacologically tractable in vitro model system comprising co-cultured primary human endothelial and vascular smooth muscle (EC-vSMC) cells [29].
Since temperature showed an obvious effect on this kind of male-male courtship behavior, we used a complementary strategy to rule out the effect of temperature on the attractiveness [16] and to confirm the implication of DA reduction in male homosexual attractiveness (the male tendency to stimulate the courtship of other males, as opposed to the male propensity to court other males).
To avoid photophobic behavior, we used a double-blind background involving the dTrpA1 1 and glass 60j mutant alleles.
To assess self-management behavior, we used a validated instrument that asks on how many of the previous 7 days the individual performed recommended activities: eating healthy foods, following a diabetic diet, exercising, self-monitoring of blood glucose, and caring for one's feet (19).
To assess the relation between CMRO2 and behavior, we used a regression method that is robust against univariate outliers in the data, as implemented using a robust linear model procedure (rlm) of the R statistical language (R Development Core Team 2009).
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To relate the neuroimaging findings to eating behavior, we used an ad libitum buffet to measure energy intake immediately after the fMRI session.
To directly examine the functional significance of podocalyxin expression on lung-colonizing tumor cell frequency and metastatic behavior, we used an in vivo competitive assay (experimental lung metastasis).
To quantitatively link the structures observed in the samples with their measured scattering behavior, we used an electromagnetic vectorial theory developed previously for the study of low-scattering media [ 18, 19].
To further quantify the pausing behavior, we used an unbiased step-finding algorithm that generated the average FRET value for each pause and its dwell time (Kerssemakers et al., 2006, Myong et al., 2007).
To further investigate motor behavior, we used an open-field paradigm together with video-assisted motion tracking (23, 24) (Fig. 3D) and compared adult TBPH−/− mutant LOF escapers with EB1-Gal4-mediated UAS-TBPH GOF flies targeting TBPH to ellipsoid body neurons of the central complex that are upper motor neurons due to their prominent role in the higher control of locomotion (25).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com