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Finally, after intraplantar injection of AM404, the biting and licking behavior was recorded for 5 min. All behavioral experiments were performed in a quiet room and evaluated by a single investigator in a blinded manner.
On experimental day 1, locomotor behavior was recorded for 2 h post-saline injection followed by sensory evoked field potential recordings after saline and three different escalating (0.6, 2.5, and 10.0 mg/kg) MPD doses.
Behavior was recorded for 8 or 9 days each Period, from 11 00 to 22 00 h.
Animals were then returned to their colony and behavior was recorded for minutes 15 30 post-injection.
Behavior was recorded for 2 min while the animals were optogenetically stimulated with 16 Hz sinusoidal light once they entered the zone where stimulus was placed56.
The fatigue S N behavior was recorded for maximum applied stresses between 300 and 500 MPa (R = 0.1, 5 Hz).
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Electroencephalograph and seizure behavior were recorded for the evaluation of the severity of seizures.
Two measures of visual search behavior were recorded for each video clip.
After that, daily weight, stool consistency, rectal bleeding and animal behavior were recorded for up to 7 days.
The number of movement of the paw and duration of the behavior were recorded for 30 s following the acetone application.
Data on smoking behavior were recorded for all attending individuals aged 15 years and above as part of this surveillance system.
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