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Behavior was recorded at 15 and 24 wk using instantaneous scan sampling.
To record tail movement, a similar setup was used in Bӧhm, Prendergast et al., 201657 where an 890 nm LED was used to illuminate the larva and behavior was recorded at 250 Hz from below through a 5x microscope objective and imaged on a camera (Basler) with a 50 mm camera objective.
At later stages, foals' behavior was recorded at pasture every 2.5 minutes for 2 hours every day for 4 or 5 consecutive days (200 250 scans per foal) [61].
Behavior was recorded at 35 frames per second for 2 min at 30 31°C.
Larval behavior was recorded at 21 frames per second on a Zeiss Stemi 2000-CS stequippedoscope equipped with an AxioCam MRm camera (Carl Zeiss AG, Jena, Germany).
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The behavioral response of pollinators to each treatment was observed for 20 min, and behavior was recorded as (1) approached at close range (hovered less than 5 cm from buds but did not touch them) or (2) touched buds.
For example, if a behavior was 'present' within one community and 'habitual' within the other community at the same field site, the behavior was recorded as 'habitual' for that field site.
Observed and interactive behaviors were recorded at 4 time points during the perioperative period: T1 "preoperative" (between 18 and 24 hours prior to surgery), T2 "between 30 min and 1 hour after the end of surgery and prior to administration of additional analgesics", T3 "approximately four hours after postoperative analgesia" and T4 "approximately 24 hours after the end of the surgery".
An average heat score was calculated for each of 28 primiparous cows in which estrous behavior was recorded for at least two consecutive estrous cycles starting from 30 days post-partum.
Ten larvae per treatment group were placed individually into 96-well plates for the challenges, and behavior was recorded for at least 20 min. All larvae were euthanized after the observation period with a lethal dose of MS-222 (tricaine methanesulfonate).
Behavior was recorded for 10 hr at a rate of 10 frames per second using a 5 megapixels CCD camera (Prosilica GC2450, 2448 × 2050 pixels, Allied Vision Technologies, Stadtroda, Germany).
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