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The phase transition behavior was checked with DSC, rheometry, and polarized light microscopy experiments.
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The catalytic behavior was checked in the acylation of three different sulfonamines: benzenesulfonamine, p-nitrobenzenesulfonamine, and p-methoxybenzene-sulfonamine with acetic acid, acetic anhydride and maleic anhydride.
Immiscibility was checked with thermal data.
Nattokinase production was checked with fibrinolytic activity.
Transfer efficiency was checked with Ponceau staining.
Read qualities was checked with FASTQC [ 50].
Integrity was checked with an agarose gel.
Reproducibility was checked with positive controls.
Integrity was checked with agarose gel electrophoresis.
The dynamic behavior of this model is being checked with the message sequence chart (MSC).
The HBS should be checked with variables that affect hygiene behaviors of students (Additional file 1).
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