Sentence examples for behavior of weak binders from inspiring English sources

They do not include other phenomena such as shear or pressure-related flow effects that could preferentially affect the behavior of weak binders and the ultimate separation performance of our microcolumns.

Here we demonstrate, in a high-throughput screening format, affinity selection of weak binders to a model target of albumin by zonal retardation chromatography.

Furthermore, co-crystallization screening allows the identification of weak binders, such as fostamatinib (provided they are soluble at 1 mM in 10% DMSO), which are otherwise typically discarded during HTS campaigns or remain undetected due to limited assay sensitivity.

The peptides have the characteristic feature of being rather weak binders with a low-affinity interaction (KD of 1 mM from previous experiments with the unmodified peptides) and may dissociate quickly.

They vary in their target-binding affinities, allowing the isolation of strong and weak binders for different applications.

JQ1 and dinaciclib were used as controls for strong and weak binders of BRD4-1, respectively.

After the incubation time L-e-S1P bio)–RNA compL-e-S1P bioimmobilized L-e-S1P bioidin- oRNAeutravidin-complexesads (Thermo Scientific) and wereed wimmobilizedon buffer to get rid of non-binders and weak binders.

The greatest problem yet to overcome is probably the mind-set of the individual researcher that weak binders are undesired and therefore of no benefit.

NMR is of particular importance for identifying weak binders for the fragment-based approach, for validation of high-throughput screening hits, and for structure-based design.

Peptides identified in the assay as pocket binders have in general shown higher affinity than those not utilizing the pocket, and all the most potent ligands (3 d, 3 f, 3 j, and 3 k, colored purple) also bind to the pocket exhibiting the highest ΔΔ Tm>4.0 K. None of the peptides designated as weak binders (in blue) showed pocket binding.

The cutoff for weak binders was set at the default value of a% rank of 2 or IC50 of 500.