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We assume a known statistical distribution for the dynamic behavior of the constructs, and show how a compile-time decision about assignment and/or ordering as well as timing can be made.
The transamidating activity and GTP binding behavior of the constructs was as would be predicted based on our knowledge of the structure and function of TG2 [10] [11], [11].
Our observations in cellular milieux are in contrast to the behavior of the constructs containing the FG-N domain in buffer alone (Hulsmann et al., 2012).
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Knowledge of the hydrolysis of each polymer block module enabled the prediction of the overall degradation behavior of the constructed copolymers.
In order to further dissect the energy flow behavior of the constructed model, representative energy characteristics (i.e., energy harvesting rate, energy consumption interval, and state probability of energy) corresponding to each component of the SEn-BS system are depicted as follows.
In conclusion, this study demonstrates that the proposed signal-to-intensity ratio method to calculate LIC by MRI is reproducible in several different 1.5 T systems (from different companies) and also that the behavior of the constructed phantom reproduces the behavior of patients with moderate or high iron overload.
For synthetic biology applications, such a minimal organism should not influence the function of any inserted genetic device (orthogonality principle) and should display a minimal biological complexity in order to ensure full predictability of the behavior of the constructed system [ 2].
Although the majority of studies to date have relied on molecular and biochemical methods to characterize the ability of tissue-construct systems to mimic the target tissues, the micro- and hence macromechanical behavior of the construct is likely to influence the efficacy of any resulting therapy.
Sequences important for the behavior of the construct are labeled and color coded: hIgλE —human Igλ enhancer; RSV rous sarcoma virus promoter; u-exon-intron-exon upstream splice cassette; tdT and DsR – Tomato and DsRed open reading frames, respectively; IRES-bsr– internal ribosome entry site followed by the blasticidin resistance open reading frame; SV40 polyA —SV40 polyadenylation signal.
Sequences important for the behavior of the construct are labeled and color coded as in Figure 1 and explained in the following: hIgHiE— human Ig heavy chain intron enhancer; UB promoter — human Ubiquitin C promoter; d-exon-intron-exon — downstream splice cassette; GFP GFP open reading frame; BGH polyA —Bovine Growth Hormone polyadenylation signal.
We initially assessed the gating behavior of these constructs by the ampicillin resistance phenotype their genes confer to E. coli.
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