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6) The homing behavior of small micromeres is intriguing and may provide a great model to study cell migration.
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Both reasons for surgically transplanting the micromere ensured the re-establishment and survival of a high percentage of small micromeres in the host embryo (Table 1 shows scoring of transplant efficacy).
We followed the migratory process at a higher cellular resolution using time-lapse to resolve the seemingly contradictory data on the movement of small micromeres during gastrulation.
No matter where they were placed, the vast majority of small micromeres transplanted to ectopic positions anywhere in the embryo made it home (Equator = 93.7% (n = 74), Animal Pole = 78.6% (n = 11), Blastocoel = 78.6% (n = 11)).
The migratory behavior of the small micromeres suggested that they underwent an EMT at the beginning of their migratory phase over the archenteron and to their coelomic pouch.
Transcription factor FoxC, however, is expressed in both the oral coelomic pouch and the small micromeres at the time of small micromere homing (Luo et al., 2012).
At the end of gastrulation, the outer layer is composed of smaller micromeres including pigment cells that give embryos a beige colour; bigger macromeres are present in the inner cell mass (Fig. 1I).
10.7554/eLife.08827.006 Figure 2. Laminin remodeling at the tip of the archenteron facilitates migration of the small micromeres to the posterior end of the coelomic pouch.
Results detail some of the complex morphogenetic movements of the small micromeres in their migratory trajectory.
In order to understand this homing ability at a systems level, we took advantage of the robust homing nature of the small micromeres to determine the GRN at work during their migration.
When control micromeres were transplanted to a control host, homing of the small micromeres occurs 100% (n = 9) of the time (B ).
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