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Furthermore, we present a detailed mechanism that explains the "on off" and "blinking" behavior of single GFP-molecules with the proposed energy-level diagrams.
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Traces show stepwise decreases in fluorescence, indicative of photobleaching of single GFP molecules.
Recently we have demonstrated optical detection of single GFP molecules using continuous-wave (CW) excitation of two-photon fluorescence [ 26].
In addition, dividing the weight w ˜ s (i ) by the known weight of a single GFP molecule (4.48210−20 g), we get an estimate of the number of GFP molecules in the bands for each strain.
TIRF microscopy imaging of single GFP-Lpd1−1250aa molecules revealed that FL Lpd localizes predominantly to the leading edge of ruffling membranes and cycles on and off the plasma membrane on a subsecond time scale.
We next tested whether Nudel, Lis1 and dynein's binding partner, affects the behavior of single dynein molecules in vitro.
(304) Classic example includes the early work of Bustamante and co-workers 5) co-workers 5co-workers, investigandng the Chuplex mechandcal behavior of single DNA molecules.
We next observed the behavior of single motor molecules on surface-immobilized microtubules in vitro using time-lapse multi-color TIRF microscopy.
To verify that the experiments are performed on the single molecule level, the typical blinking behavior of single molecules is confirmed in the wide-field configuration with millisecond integration times.
Recent advances in optical techniques have permitted researchers to probe the behavior of single molecules in a solid or liquid sample.
The absence of a significance difference in the apparent diffusion coefficients suggests that our labeling strategy does not alter the behavior of single molecules.
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