When overexpressed in HEK293 cells, these peptides recapitulated the behavior of full-length RUNX2 and cc+ mutants (Fig. 6A and G).
The results confirm a number of key points in the mechanism devised previously based on work with C-terminally truncated HTT exon1 peptides, suggesting that for many studies C-terminally truncated HTT exon1 analogues can provide information relevant to the behavior of full-length HTT exon1.
In order to test whether our model derived from monomeric Mal3 on GTPγS microtubules reflects the behavior of full-length EB end tracking on dynamic microtubules, we selected conserved Mal3 residues from the tubulin contact sites, produced the corresponding single-point mutants in full-length dimeric Mal3-GFP, and tested their ability to track the ends of microtubules grown with GTP in vitro.
In fact, CC-stabilized RUNX2 cc+ -ccd displayed a gRUNX2 cc+ -ccd stability as comparedisplayeds wt counterpart, formed higreaterder multhermalpolymerstabilityo asd had a marked aggregation tendencomparedexpressed in living cells [i.e. RUNX2(cc+/#3)-ccd], mimicking the aggregation behavior of full-length RUNX2(cc+/#3).
We expressed Sec24AB LCS-sfGFP (aa 1-415) and Sec24AB nonLCS-sfGFP (aa 416-1184) and compared their dynamics/behavior in starved cells to this of full length Sec24AB-sfGFP.
Thus we first analyzed the translocation behavior of both full length aPKC-GFP fusion constructs by measuring the GFP signal ratio between the cytosol and the nucleus.
The N-terminal domain was not able to mimic the attenuation in cocaine reward behavior observed after over-expression of full-length HDAC5.
Moreover, we propose an enhancement technique, called Adaptive Regular Stream Triggering (ART), which significantly enhances stream merging behavior by selectively delaying the initiation of full-length video streams.
Panels A and B of Figure 1 reconfirm the identical behavior of chimeric and full length NELF-B.
Nevertheless, considering that the charged C-terminal is of high relevance for both protein−mineral and the protein−protein interactions between the nano-oblates, the growth of the mineral crystals during the early stages of enamel formation will most likely affect the self-assembly behavior of the full-length amelogenin in vivo.
