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Our findings indicate that fibroblasts in premalignant microenvironment are involved in premalignant transformation of mammary epithelium and play a role in control the malignant behavior of epithelial cells.
The activation of fibroblasts is driven by decreased miR-200b and miR-200c, which trigger malignant behavior of epithelial cells growth and polarity changes.
This suggests that the presence and relative locations of cells, ECM, and matrix-free (or cell-free) space are very essential for the expected behavior of epithelial cells.
Most experimental investigations employ a two-dimensional substrate; however, to gain further insight into the behavior of epithelial cells in-vivo, we must switch from 2D to a 3D model.
We have recently shown that the collective migratory behavior of epithelial cells was strongly affected by the geometrical constraints of the environment.
For all cell combination experiments, fibroblasts were used at a 2.5:1 ratio to promote the most aggressive behavior of epithelial cells (data not shown).
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Current fate mapping and imaging platforms are limited in their ability to capture dynamic behaviors of epithelial cells.
Title A 3D Culture Model to Study How Fluid Pressure and Flow Affect the Behavior of Aggregates of Epithelial Cells.
A 3D Culture Model to Study How Fluid Pressure and Flow Affect the Behavior of Aggregates of Epithelial Cells.
Here we describe a protocol to determine the effects of interstitial fluid flow on the migratory behavior of an aggregate of epithelial cells in a three-dimensional (3D) culture model.
Further studies are currently in progress to elucidate the biological behavior and genetic profile of epithelial cells immediately subjacent to these SMA-negative ME cells.
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