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The hygienic behavior of each hive was estimated as a percentage of the dead larvae removed by the worker bees.
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The behavior of each queen in a hive was monitored by a different observer.
Half of the squares on both sides of each hive and the bridge were observed visually each day (138 squares per hive per day).
The entrance of each hive was covered with a queen excluder – a grid that allows workers to exit and enter, but confines the larger queens within the hive.
A mesh screen was placed over the entrance of each hive to facilitate the capture of returning foragers.
Briefly, 100 mL of liquid nitrogen was poured on two circles (15 cm diameter) within the brood area of each hive (7 day old larvae).
Scientists have long studied the intelligent "emergent behavior" of hive-style animals.
This was done by selecting appropriate matched controls within one month of age for each HIVe case.
Hive sounds are as noisy as comb vibrations, since there are several types of sounds caused by wing vibrations produced by the behavior of nestmates while maintaining the hive.
Researchers at Harvard are trying to develop "robobees," which would mimic the mechanics and the hive behavior of real bees, whether in the service of pollination or of stealth surveillance.
After making a second queen, about half of the 60,000 bees in each hive left in swarms to find a new home.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com