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Further, KalignP is more flexible than Kalign2, as researchers can modify the behavior of alignment using other knowledge.
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Although we have not examined for ultramicro inversions within the genome alignment generated by local alignments other than blastz, differences in the ultramicro inversions between different alignment algorithms may be helpful in verifying the behavior of the alignment algorithms involving ultramicro inversions either erroneously aligned or excluded from the local alignments.
Predicting the behavior of a possible alignment method at this scale may not lead to reliable conclusions.
Functional assignments for short-read metagenomic data pose a significant computational challenge due to perceived unpredictability of alignment behavior and the inability to infer useful functional information from translated protein-fragments/peptides.
To address this problem, we have examined the predictability of short peptide alignments by systematically studying alignment behavior of large sets of short peptides generated from well-characterized proteins as well as hypothetical proteins in the KEGG database.
Because glial cells are crucial to the proper function and survival of neuronal cells and because they support and direct neurite growth, the behavior and alignment of SGSCs was also measured and compared on unpatterned and uni- and multidirectional micropatterns.
Since a significant proportion of metagenomic data routinely falls into this hard case category, it is likely that alignment behavior of peptides from hard case proteins could confound quantification when using all alignment information of peptides in conjunction with frequency-based weighting.
Given the dearth of empirically-derived data on the alignment behavior of peptides that could even be used to model thresholds for SSMs, we were motivated to systematically study the actual alignment behavior of short protein fragments.
Spiral ganglion Schwann cells (SGSCs), which provide trophic support to SGNs, mirror the alignment behavior of SGN neurites on each pattern type.
Alignment behavior of blast-hits of peptide to parental and non-parental proteins overlaps significantly irrespective of the length of the peptides (Test case type1_11-81aa, Additional file 1: Figure S1-S8).
The alignment behavior of short peptides was examined in detail by Sander and Schneider [ 34] and Rost [ 35] who showed that segments of proteins having alignment lengths between 10 and 80 aa are structural homologs provided the corresponding minimum alignment identities are 40 80 %.
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