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We found that exonization of transposed elements is biased towards the beginning of the coding sequence in both human and mouse genes.
Here we show that exonization of transposed elements is biased towards the beginning of the coding sequence in both human and mouse genes.
At the beginning of the coding region (first 17 positions, excluding start codon), the structural differences are marginal or non significant (Figure 2a).
The PSMD8 and RPA39 promoters (wild type and TISU mutant) and the beginning of the coding sequences were fused to the green fluorescent protein (GFP) such that the ATG of TISU is in frame with that of the GFP (Fig. 6B).
We found the "translational ramp" also reported by other authors at the beginning of the coding regions.
Here, no sequence information about the 3'end of this promoter as well as the beginning of the coding sequence was available.
However, PTCs at the beginning of the coding region can trigger strong NMD also in genes without introns; similarly to what has been reported in other systems.
The regulatory mechanism would operate through the recognition of sequence elements located at the beginning of the coding region and in the 3´ UTR of the tubulin genes [ 31].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com