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At the beginning of the behavioral experiments the subjects (n = 16 in each genotype) were 4 5 months old.
One hour elapsed between the injections of substances and the beginning of the behavioral tests.
Better test scores of the latter were, however, not immediately evident at the beginning of the behavioral tests.
After weaning, the offspring were separated by sex and housed in group cages (four or five per cage) until the beginning of the behavioral tests.
Two days before the beginning of the behavioral training, the rats were weighed and food availability was reduced to achieve 85% of the previously determined body weight.
All frames at the beginning of the behavioral quantification are marked as FORWARD, unless they meet the criteria of another behavioral marker, namely an OMEGA TURN, a REVERSAL, or a PAUSE.
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At the beginning of this new set of blocks as well as at the very beginning of the whole behavioral training session the "correct" associations were presented to further facilitate the training process.
One week before the beginning of behavioral tests, the rats were housed one per cage and were handled once a day for 5 days.
Their body weights ranged from 20 to 25 g at the beginning of behavioral training, and the mice were maintained at greater than 90% of their ordinary body weights with free feeding throughout the training period.
The tests were conducted using a Perspex board (10 cm × 20 cm) containing 50 randomly distributed slots, each filled with a food pellet at the beginning of the test (home-made behavioral apparatus).
Finally, we began exploring the molecular basis of the behavioral connections between Drep-2 and both mGluR signaling and FMRP.
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