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Although under these conditions Hmo1 depletion also occurred efficiently, it did not anymore correlate with reduced SCJ levels (Supplementary Fig S2), in contrast to its effect at the beginning of replication (Fig 2B, 60 120 min panels).
At the beginning of replication, ATP binds DnaA, resulting in a large oligomeric complex consisting of DnaA monomers bound to a series of 9-mer consensus elements termed DnaA boxes.
sgs1Δ hmo1-AID cells grow normally, but Hmo1 depletion at the beginning of replication correlated with a decrease in the amount of SCJs (Fig 2B, 60 120 min panels), which gradually increased following prolonged MMS treatment (Fig 2B, 180 240 min panels).
Conditional depletion of Ddc2 at the beginning of replication, using the Tc-DDC2-AID allele described above, led to abolishment of MMS-induced Rad53 activation and a drastic decrease in the DDT-associated SCJs (see Figure 6), whereas checkpoint inactivation later on did not affect the processing kinetics of error-free DDT intermediates or the late resolution (see Figure 1A).
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Because viral replication is both curtailed and delayed in n406 infected cells, the smaller foci seen at 8 h in n406 infections, probably reflect the timing of the beginning of DNA replication.
Thus, nuclear accumulation of FBPase in the APC-synchronized cells indicates that the enzyme is transported into the nucleus prior to the beginning of DNA replication, at the G1/S boundary of the cell cycle.
The highest ratios and numbers of cytokine genes are induced in the early, active infective state, in which C. trachomatis is at the beginning of the replication cycle and metabolically active (4 h).
At the beginning of DNA replication, GFP-Pcn1 foci formed throughout the entire nucleus except for the rDNA region; the GFP-Pcn1 signals in the nucleus then became dim and accumulated only in the rDNA region at the end of DNA replication (Fig. 2A).
Since old flagella pre-exist in cells which were affected by RNAi at the beginning of cell replication, the real percentage of silenced cells is probably higher than 60%%, which is confirmed by the fact that it built up to more than 74 % at time point 22 h.
In the beginning of this period replication in mitochondria are almost arrested, and the initial pool of mitochondria is distributed between the dividing cells.
Synchronized cultures of WT and PPLP2 parasites showed the same percentage of infected erythrocytes at the beginning of the second replication cycle (day 3) in synchronized cultures with the adjusted parasitemia on day 1 (Fig. 4A), suggesting the same efficiency of parasite replication in WT and PPLP2 parasites.
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