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The beginning of heating season is a time when carbon monoxide poses a threat.
(a) At the beginning of heating, (b) 1 min, (c) 3 min, and (d) 5 min.
Figure 4a shows the beginning of heating, and the time increases from Figure 4b to Figure 4d.
The thermal picture on the left represents the surface temperature at the beginning of heating, the right picture after about 4 minutes.
The performance of the double tube system was similar to that for the U-tube system at the beginning of heating operation, while the former was lower than the latter as days went by.
Thus, the time temperature history identical to that measured at slab bottom during the furnace testing of Specimen HD12 (Lim and Wade 2002) is applied in analysis at slab bottom surface while the slab top surface has room temperature at the beginning of heating.
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We found that HSP-16 proteins were first detected 60 minutes after the beginning of heat shock.
The heat stimuli were applied to both the injection site and the corresponding area of the contralateral paw, and the latency was determined as the duration from the beginning of heat stimuli to the occurrence of a marked withdrawal reflex.
As depicted in Figure 4B, mRNA levels of HSP-4, -16.1, -16.49, F44E5.5 and C12C8.1 were up-regulated in atx-3 animals at 15 minutes after the beginning of heat shock until at least 30 minutes after the stimulus.
Interestingly, HSP-16.2 was not up-regulated in atx-3 mutants under these conditions and was even down-regulated 60 minutes after the beginning of heat shock (Figure 4A, B).
At the protein level, atx-3 animals expressed higher levels of HSP-16 than controls 90 minutes after the beginning of heat shock, and these proteins remained up-regulated at all recovery time-points except at 24 h, when the HSP-16 levels were close to those of wild-type (Figure 5A, B).
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