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Furthermore, the deepest crack at the beginning of every experiment was found to slow or stop growing, and was then surpassed by another crack that eventually penetrated through the sample.
To process the behavioral experiments, a human analyst watched the beginning of every experiment and skipped the video to the moment when the captured ant is released (the recording of experiment started moments before the release so as to avoid losing any movement).
These sessions were carried out before the subjects entered the scanner as well as during the anatomical scan (collected at the beginning of every experiment).
At the beginning of every experiment we indeed checked that the subject's blood glucose level was at this range, while later the subject received a sweetened drink and the level was changed.
To calculate the corrected FRET ratio, we subtracted the bleedthrough of the donor fluorescence (CFP) into the acceptor (YFP) channel using the equation: FRET ratio = (YFP − 0.63 × CFP /CFP, where 0.63 is a bleedthrough coefficient measured in cells expressing CFP only and normalized the values to the initial FRET ratio recorded at the beginning of every experiment.
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Before the beginning of the experiment, every candidate was tested individually for developmental dyscalculia, dyslexia, IQ and ADHD.
were administered at the beginning of the experiment and every six hours after until the animal was returned to its home cage (the first day of each experiment typically lasted 8 to 10 hours and the second day typically lasted 2 to 4 hours).
Individuals were weighed (± 0.01 g) at the beginning of the experiment and every 20 minutes to record loss of water.
Plant height (i.e. distance from the soil surface to the apical node of the tallest shoot in each pot) was measured at the beginning of the experiment and every second or third week.
The samples for analyses were harvested at the beginning of the experiment and then every 6 days.
Cell viability was assessed using Trypan blue (Merck, Darmstadt, Germany) exclusion in every experimental set up, at the beginning and at the end of every experiment and determined to be 95 to 100%.
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