Meanwhile, Rep reached a higher value at the beginning of each load cycle.
One minute after the beginning of each experiment, cells were loaded with the potentiometric dye TMRM (Sigma-Aldrich) in Krebs-Ringer at a final concentration of 30 nM at 37°C.
The algometer was calibrated before the beginning of each test using a load of 100 kPa.
The results show that the pop-in behavior occurs at the beginning of the loading process.
The pressure gradient is quite uniform at the beginning of the loading, when the fracture is wide open (Fig. 7a).
At the beginning of the loading procedure, tensile, compressive, and shear stresses reached the minimum at about 65 mm away from the middle of the beam.
The tensile behaviour of woven reinforcements is specific because the undulations due to weaving lead to strong non-linearities at the beginning of the loading.
The stress strain state of the cross section was investigated from the beginning of the loading up to the stage exceeding ultimate limit state.
The nonlinear part at the beginning of the loading in Fig. 12 is caused by the same mechanism as the nonlinearity in the case of the elastic rock, i.e. an increase in the contact area.
In addition, besides the Rb effects, the timing of the increased Cyclin E expression correlates nicely with the beginning of MCM loading, and Cyclin E is also known to elicit at least part of its cell cycle control through facilitation of MCM loading via phosphorylation of Cdc6 [17].
