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As a control, prior to the beginning of each assay an aliquot was taken and was incubated until the end of the assay (without the enzyme).
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We ran quality control standards at the beginning and end of each assay.
Quality control samples containing endogenous cortisol were assayed in duplicates at the beginning and end of each assay.
Pooled bovine whole milk samples were used as controls at the beginning and end of each assay run.
At the beginning of the assay, each tube contained ~12,000 healthy cells in a total volume of 10 ml.
At the beginning of the assay, each well contained approximately 4,000 cells, which corresponds to 250 300 colonies.
At the beginning of the assay, each well contained approximately 1,600 cells, which corresponds to 50 70 colonies, in a total VM volume of 1 ml.
Processed images could discriminate perfectly between positive and negative attractant responses at different periods of time from the beginning of the assay.
Notably, 22C4 scFv only inhibited aggregation completely when added to Aβ1-42 athehe beginning of the assay.
In some experiments cells were treated with cytochalasin D (10 µM) or latrunculin B (50 nM) before the beginning of the assay.
Fold expansion (FE) was calculated as the number of offspring cells at the end of the assay divided by the number of cells at the beginning of the assay: FE = (N (1)+N (2)+.+N (m))/N estimated (0), where N estimated (0) = N (0)+N (1)/2+N (2)/4+.+N (m−1)/2m−1.
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CEO of Professional Science Editing for Scientists @ prosciediting.com