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Four days after transfection, we began selection with methotrexate (MTX) as described previously (Cherbas et al. 1994).
Two days later, we began selection in Puromycin (1 μg/ml concentration in media) for one month, and then PCR screened resistant cells for GRIM.miLacZoff presence.
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All of this without even beginning selection and genetic improvement programmes, which in a few years, with a fraction of what is spent in agricultural improvement, can lead to tremendous advances".
The initial concentrations of docetaxel and paclitaxel used to begin selection (dose 1) were 0.51 and 0.56 n M, respectively.
Target cells were infected at 50% confluence by overnight incubation in virus-containing medium, and were cultured for a further 24 h in full growth medium before beginning selection.
More than 100 potential jurors began the selection process in state court.
Ebury Press (publishers of the People's Manifesto) agreed to fund a candidate, and so we began the selection process.
After 2 days postinfection, we began the selection with 400 μg/ml of G418 (active drug, GIBCO) in RPMI1640.
Therefore, breeders began recurrent selection of o2 lines with high lysine and a hard endosperm, called Quality Protein Maize [ 10].
In 1926, a stud book was begun, and selection processes were developed to select horses for use in draft capacities.
Since selection began, no substantial improvement in the selection environment of line V was made (Baselga, personal communication).
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