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Unexpectedly, de novo expression of Muc5ac was observed just before worm expulsion in the Muc2 KO mice and resistant mouse models, but not in the susceptible models.
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Plates were incubated for at least 1 hr before worms were added.
The bacteria were grown at room temperature for 1 d before worms were transferred onto the plates.
Plates were incubated overnight at room temperature before worms were added.
We picked worms in the L4 larval stage and cultured them for 24 hr before worms were fixed and stained with SYP-1 and HIM-3 IF.
Before worms were fed with E. coli strains, 1 mM IPTG was treated to induce ds RNA transcription of the target genes.
This was used to seed plates, which were left at room temperature for 2 days to induce double-stranded RNA synthesis before worms were added.
Plates were typically seeded 2 3 days in advance to allow the bacterial lawns to dry out before worms were put on the plates.
I'd never seen a grass snake or slow worm before and was excited that I might see them.
Possibly the patch that fixed it - which appeared before the worm - gave its writer a clue to what to create.
Years before the worm hit Iran, Washington had become deeply worried about the vulnerability of the millions of computers that run everything in the United States from bank transactions to the power grid.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com