Sentence examples for before with minor modifications from inspiring English sources

Cell isolation was performed as described before, with minor modifications [22].

Intracellular staining of WR protein was performed as described before with minor modifications [18].

Spectral characterization was performed as described before with minor modifications [10].

As VE-cad is particularly sensitive to trypsin in absence of Ca2+, mono-cultured HUVEC and co-cultured HUVEC were detached in a way described before with minor modifications to maximally preserve VE-cad at the cell surface and to avoid its degradation [5].

Western blot analyses for human C3 deposition on reperfused ischemic tissue samples and healthy controls was performed as described before, with minor modifications.[4] Jejunal samples were homogenized in lysis buffer (200 mM NaCl, 10 mM Tris base, 5 mM EDTA, 10% Glycerin, 1 mM PMSF, 0.1 U/ml Aprotinin and 1 µg/ml Leupeptin).

Cultures were OGD as described before, with minor modifications.

High speed centrifugations were performed as described before [39], with minor modifications.

ChIPs for TAP-tagged Tup1 were performed as before [17] with minor modifications.

The global carbon assimilation profiles were evaluated by using a Biolog FF MicroPlate (Biolog, Inc., Hayward, CA) following a protocol described before (32) with minor modifications; the inoculum was prepared from cultivations on MEX plates incubated at 30°C.

Histochemical staining for 8-oxo-dG (1/300 dilution, Cosmo Bio Co., Japan) was performed as described before [25] with minor modifications: endogenous peroxidase activities were eliminated by incubation of the tissue samples in methanol 3% H2O2 for 30 min and the Tyramide Signal Amplification kit (TSA, Amersham) was used according to the manufacturer's instructions.

The MS was operated in the method described before [ 39] with minor modifications.