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T84 cells (15 × 150 mm dishes) were plated at 100% confluent density at 18 hours before utilization for biochemistry.
This was achieved by trypsinizing and re-plating confluent plates of cells at ~18 hours before utilization for biochemistry.
T84 cells (15 × 150 mm dishes) were grown to 100% confluent density and maintained for one week post-confluency before utilization for biochemistry.
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Acetone (extra pure, Merck) and N,N′-dimethylformamide (DMF) (pure analytical, Reakhim) were dried for 48 h before utilization with activated molecular sieves (0.3 nm, Merck).
N,N′-dimethylformamide (DMF) (pure analytical, Reakhim) was dried for 48 h before utilization with activated molecular sieves (0.3 nm, Merck).
Before utilization, the resin was treated with 4% NaOH for 24 h, then washed with double-distilled water.
Due to the low burning temperatures at 800 900 °C, FBC fly ash is not suitable for use as pozzolan in concrete mixture and requires proper improvement before utilization.
Before utilization in real-time RT-PCR experiments, all primers were tested by genomic PCR for their specificity and reproducibility using total genomic DNA as a template.
IGCC is promising because coal is gasified before utilization.
All constructs were verified by nucleotide sequencing before utilization.
More specifically, we were interested in examining the rate of service utilization for psychiatric reasons and suicidality before suicide.
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