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Strains were cultured for 2-3 generations before using for lifespan analysis.
The strain was cultured for 2 3 generations before using for lifespan analysis.
Water samples were boiled, treated with concentrated nitric acid and then filtered to remove organic particulate matter before using for the analysis.
In addition to quantity issues, the spring water quality was also reported to be declining due to anthropogenic factors and needs to be treated before using for domestic purposes (Nair et al. 2015).
Since TWEAK-Tg mice were generated in B6D2F1 background, these mice were crossed with C57BL/6 mice for 7 generations before using for this study.
Defensins as well as peptides were dissolved in sterile ddH2O and filtered through 0.22 µM syringe filter before using for in vitro antifungal activity assays.
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Phospholipid-modified Fe3O4 nanoparticles were washed three times with the buffer solution of interest before used for experiments.
Recombinant E. coli cells were treated by freezing at −20 °C and heating at 53 °C before used for bioconversion.
Isolated monocytes were counted using CASY technology and washed once with serum-free RPMI-1640 medium before used for adhesion assay.
Finally, the powder was calcined in air (air flow: 30 mL/min) for 30 min at 550 °C before used for SWCNTs growth.
The MnZn ferrite thin film was deposited on the p-Si by magnetron sputtering, which was annealed before used for GFET fabrication.
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