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6% TCA in DCE (w/v): Detritylation solution was prepared before use by dissolving TCA (6 g) in dry DCE (100 ml).
The saponin adjuvant was prepared immediately before use, by dissolving saponin powder (Sigma, ref. S4521, batch 010 M7015) to obtain the desired amount per dose.
Solution of DITPA was prepared immediately before use by dissolving the powder in 0.1 N NaOH and diluting with 0.9% saline (pH 9)[ 40] (not more than 0.1 ml was injected).
They were freshly prepared before use by dissolving them in a mixture of 30% (v v−1) polyethylene glycol 400, 20% (v v−1) ethanol and 50% (v v−1) H2O.
The in vitro studies of adipogenic and osteogenic differentiations were performed according to the methods described previously by Karaoz et al. STZ was prepared 1 min before use by dissolving in 0.1 mol/l sodium citrate (pH 4.5) at 4°C a concentration of 200 mmol/l.
Similar(53)
Different NFA concentrations were prepared immediately before use by diluting niflumic acid that was dissolved in dimethyl sulfoxide (DMSO) in the standard bath solution.
For in vivo studies, PTX was dissolved by adding absolute ethanol and Cremophor ELP (both 5% of the final volume) (Polizzi et al, 1999), whereas IDN 5390, dissolved in Polysorbate 80, was diluted just before use by adding 0.9% NaCl to the final concentration of 10% v v−1 (Pratesi et al, 2003).
Chromatography gels were washed before use by 0.5% Triton X-100 (TX-100) (Merck), water and equilibrated with buffer. 1 μmole egg-PC dissolved in CHCl3 was placed in a 10 mL glass tube.
The drugs were prepared fresh before each experiment, by dissolving in saline, and were kept cold and protected from light until used.
NAC was prepared just before the experiments by dissolving the powders in MilliQ water.
Immediately before use, MMC was dissolved in 0.9% sodium chloride to the appropriate concentrations.
More suggestions(14)
before use by bubbling
before use by suspending
before use by being
before use by mixing
before use by filling
before use by washing
before use by opening
before use by holding
before use by using
before use by following
before injection by dissolving
before use by injecting
before use by passing
before use by scrubbing
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