All cell lines were banked and passaged for less than 6 months before use, and were maintained in a humidified atmosphere containing 5% CO2 at 37°C in RPMI-1640 (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% FBS; Welgene, Inc., Daegue, South Korea) and 10 μg/mL gentamicin (Cellgro, Manassas, VA, USA).
Eben Amstrong,a biomedical engineer, not only inspects and maintains equipment before it ships, he travels to recipient hospitals several times a year to train people to use and maintain it.
3. Use and maintain sprinklers properly.
Animals were housed in vivarium minimum of 2 days before use, maintained on a 12/12 hour day-night cycle, and are given food and water ad libitum.
To maintain its sparkling personality, even in the short term, the water must be very cold (the smallest increase in temperature reduces the ability of a liquid to hold gas) and the bottle opened just before use to maintain the carbon dioxide level.
Spayd, who had most recently been editor of the Columbia Journalism Review, rarely used Twitter before taking the job and maintained much less of a presence on social media as public editor. .
NIH-3T3 fibroblast (N3Fs, originally obtained from ATCC) were pre-conditioned in media containing 10% fetal bovine serum (FBS) during serially cultivating for at least 22 passages [28], [29] before the use for 3D matrix production and maintained in Dulbecco's modified Eagle's medium supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin.
Cells were used at passages 2 6 and maintained in 1% FCS 24 h before the experiments.
HEK293T cells were transiently transfected with wild-type and individual mKv1.3 mutants using Lipofectamine 2000 (Invitrogen) and maintained in DMEM at 37°C for 24 hrs before experiments.
