Sentence examples for before usage for from inspiring English sources
OPM-2 cells were lentivirally transfected to express eGFP and propagated in the presence of blasticidin (2.5 μg/mL, Invitrogen) before usage for in vivo experiments.
Polyclonal antibodies were generated by immunization of rabbits with synthesized polypeptides corresponding to the indicated domains (details about peptide sequences are available on request) and affinity purified before usage for immunoblotting.
The excessive ammonium was removed through struvite precipitation before usage as substrate for PHAs production.
It's understood the advances Spotify paid to major labels before launching exceeded usage for the deal period (the labels also own shares in the service), and artist managers have confirmed to me that at least one of the majors paid through the excess advance royalties to its artists, basing the amount on the artist's share of streams during that period.
An understanding of the physical, chemical and biological properties of water is crucial before it's usage for given purposes.
Although the howitzer could equally be fired from the chassis, the vehicle was designed to carry the artillery piece to a firing emplacement for removal before usage.
The enzyme was dissolved in buffer solution (136 mM NaCl, 0.1 mM CaCl2) for injection before usage.
Fishes were acclimatized for 1 week before usage, in order to accumulate fish waste to enable the supply of nutrients for romaine lettuce.
Three different FCC catalysts were prepared for comparison: Cat1: 30 % USY + 60 % Kaolin + 10 % Silica gel; Cat2: 30 % USY + 10%% microporous SAPO-11 + 50 % Kaolin + 10 % Silica gel; Cat3: 30 % USY + 10%meso-SAPO-11meso-SAPO-11meso-SAPO-11+50%ca gel; The catalysts were hydrothermally treated at 760 °C for 4 h before usage.
Therefore glassware was heat-sterilized at 180°C at least for 3 h and the whole setup was flushed with 1 M NaOH for 1 h before usage.
After coupling, 0.1% normal mouse serum was used for 2 hours before usage to capture the unbound GAMAP, after which the antibody solution was used on the slides.
