The slide was then transferred to 1 × TBE (10 mM Tris (pH 7.5), 1 mM EDTA) for 5 min to neutralize before undergoing electrophoresis in a horizontal chamber in fresh 1 × TBE at 1 V/cm for 10 min.
Sections (4 μm) from this block were mounted onto slides before they were deparaffinized, rehydrated, and microwave treated in target retrieval solution pH 9.9 (Dako, Glostrup, Denmark), before undergoing processing in an automated immunostainer (Techmate 500; Dako, Copenhagen, Denmark), using the Envision software (Dako, Glostrup, Denmark).
October 2015 Diagnosed with lung cancer, having been a heavy smoker before undergoing surgery in 1991.
The painting will become the centrepiece of the Queen's House when it reopens on 11 October before undergoing conservation in 2017.
The popular Cardiff stalwart revealed he almost quit football because of his "frustrating nightmare" before undergoing treatment in the United States.
Patients with a glycosylated hemoglobin level of 11.5% or greater should be more optimally controlled before undergoing implantation in an effort to avoid infectious complications.
All 15 patients underwent mammography, basal ultrasound, and CE ultrasound, before undergoing surgery, in order to study the appearance of the microcirculation of these lesions and to compare these data with the surgical specimens.
Motte saved 42 games for the Cardinals in 2012 before undergoing Tommy John surgery in 2013.
Samples were then washed in 0.1 M sodium cacodylate before undergoing secondary fixation in 2% osmium tetroxide, 1.5% potassium ferricyanide and 0.1 M sodium.
The fabled club has been the site of professional and collegiate tournaments, in addition to co-hosting the state amateur championship before undergoing major renovations in 2009.
