Sentence examples for before treating with from inspiring English sources

Cells were incubated in cystine-methionine deficient media overnight before treating with oxidants.

Cells were incubated with BSO (100 µM) overnight before treating with oxidants.

Cells were typically grown to 80 90% confluency before treating with trypsin-EDTA (Gibco, 15400) and splitting.

Eight micrograms seedling leaf DNA from individual plants was restricted with SspI and ScaI and purified with phenol-chloroform before treating with sodium bisulfite [49], [56].

Accordingly, cultures were synchronized in G2 and released for approximately 30 minutes to proceed through the first synchronous mitosis before treating with CPT in the subsequent S phase.

For the mRNA expression analyses, MCF7, HCT116 and GIMEN cell lines were seeded onto 100 mm Petri dishes and allowed to reach 70 80% of confluence before treating with different drugs as described in the figures.

To assess the memory population after OX40 ligation during priming, mice were immunised as before, treated with control or agonistic anti-OX40 Abs at 1 dpi and at 28 dpi given 2W1S peptide in vivo and analysed 4 hours later.

Sintchenko et al suggested that low-risk patients with ILI should be tested before treated with antivirals and that high-risk patients would benefit from prompt treatment [21].

Further, OA was surgically induced in the right knees of adult Wistar rats before treated with dPGS to answer the following questions.

ECs (1 × 10 cells per well) in 96-well plate were starved in basal CSC medium containing 2% FBS overnight before treated with 1  μM ISM and 15 ng/ml VEGF for 24 h.

On the other hand, NK cells which express CD56+CD16−/+ were also isolated from fresh PBMC before treated with R. korthalsii methanol extract for a better understanding of the immunoregulatory effect contributed by direct interaction between the extract and NK cells.