Sentence examples for before treating the cells from inspiring English sources

The spermatozoa were washed several times with PBS, before treating the cells with streptavidin-peroxidase for 30 min. The cells were observed under a light microscope after staining with AEC kit.

SSZ and DFMO were diluted with culture medium before treating the cells.

Substances were dissolved in DMSO and added to cells shortly before treating the cells with biological stimulators.

We plated 5× 10 cells/well in 96 well plates for 12 h before treating the cells with α-synuclein (20 μM) for additional 12 h.

To examine the involvement of the ER axis, we pretreated mDCs with an ER antagonist, ICI182,780 (10 μM; Sigma), 1 hr before treating the cells with NP or 4-OP.

To test the requirement of shugoshin and condensin for sister kinetochore bias, we allowed cells to progress from G1 into the cell cycle for 1.5 hr to allow SPB separation, before treating the cells with nocodazole for 30 min. Subsequently, nocodazole was washed out and we simultaneously began filming.

To further confirm the critical role of endogenous ecto-phosphatases, we added ALP (at 10, 1.0, 0.01 0.01, or 0.001 U/mL), as an exogenous enzyme, to DDD- 1P instantly before treating the HeLa cells.

We therefore, recorded fluorescence intensity profiles over selected plasmalemmata-cell wall sections before and after treating the cells for 15 to 30 min with 10 nM BL.

Identical results were obtained with a different crowding agent, replacing the PEG-8000 and trehalose with 10% PVP and treating the cells as before.

Hemin (Sigma-Aldrich, St . Louis MA) was dissolved in 0.25M NaOH solution, pH was adjusted to 7.6 with HCl and the solution was then filter-sterilized before treating endothelial cells.

To investigate further the molecular pathways involved in GSIXII induction of cell death, we performed siRNA-based experiments against Noxa, Bim, or Puma before treating cells with GSIXII.