Sentence examples for before treated with from inspiring English sources

After washing with TBST for 10 min three times, the membrane was incubated with appropriate secondary antibody at room temperature for 1 h, washed three times with TBST as before, treated with SuperSignal (Pierce) detection reagents, and exposed to Kodak XAR-5 film.

To assess the memory population after OX40 ligation during priming, mice were immunised as before, treated with control or agonistic anti-OX40 Abs at 1 dpi and at 28 dpi given 2W1S peptide in vivo and analysed 4 hours later.

Sintchenko et al suggested that low-risk patients with ILI should be tested before treated with antivirals and that high-risk patients would benefit from prompt treatment [21].

Further, OA was surgically induced in the right knees of adult Wistar rats before treated with dPGS to answer the following questions.

ECs (1 × 10 cells per well) in 96-well plate were starved in basal CSC medium containing 2% FBS overnight before treated with 1  μM ISM and 15 ng/ml VEGF for 24 h.

MCF-7 eGFP-MAP1LC3B cells were seeded in 96-well plates (NUNC, 167008) and cultured for 72 h before treated with control medium or 100 U/ml of IFNB1 for an additional 24 h period, whereas rapamycin (200 nM) was added 2 h prior to fixation in 4% formaldehyde.

Cells were incubated with BSO (100 µM) overnight before treating with oxidants.

Cells were typically grown to 80 90% confluency before treating with trypsin-EDTA (Gibco, 15400) and splitting.

Cells were incubated in cystine-methionine deficient media overnight before treating with oxidants.

Eight micrograms seedling leaf DNA from individual plants was restricted with SspI and ScaI and purified with phenol-chloroform before treating with sodium bisulfite [49], [56].

Accordingly, cultures were synchronized in G2 and released for approximately 30 minutes to proceed through the first synchronous mitosis before treating with CPT in the subsequent S phase.