Sentence examples for before transient from inspiring English sources

After 24 hours of knockdown, cells were passaged to a 6-well plate before transient transfection of reporter plasmids.

Retroviral transduction of E6 before transient transfection of immortalized FHL2−/− cell lines with H-rasV12 resulted in efficient colony formation (see Fig. 3C), thus allowing efficient transformation of these cells.

HeLa cells were seeded in a 24-well plate before transient transfection with 0.4 µg of either empty pSuper plasmid, a non-silencing shRNA, or Tat-SF1(A) and Tat-SF1(B) in combination using Lipofectamine 2000.

NS309 (0.2 and 2 mg/kg) was applied intraperitoneally 30 min before transient focal cerebral ischemia.

30 Before transient hypoxia-ischaemia, baseline data were acquired after stabilisation of the animal in the MRS system.

Briefly, 1 × 10 cells were seeded per well in a 24-well plate before transient transfection with the construct TOPflash or FOPflash reporter plasmid (Millipore, Billerica, MA, USA).

Cells were seeded on poly- l-lysine-coated 22 mm glass coverslips (Marienfeld, Lauda-Königshofen, Germany) at 0.25×10 cells/coverslip 2 days before the experiment or at 0.4×10 cells/coverslip 1 day before the transient transfection and 3 days before the experiment.

Cells were seeded at 0.4×10 cells/well in poly- l-lysine-coated 12-well plates 1 day before the transient transfection and 3 days before the experiment.

Briefly, spin-echo multi-slice T1-weighted imaging was performed with the following scanning parameters: TR 600 ms; TE 10 ms, matrix size = 256×192, FOV 25 mm×25 mm and 1 transient, before and at various times after intravenous (IV) infusion of manganese chloride (62.3 mM, 5 µl/g MnCl2.4H2O, Sigma-Aldrich, Poole, U. K).

If we do not utilize the OM-LSA estimator before the transient noise reduction, the background noise somewhat disturbs the pitch estimation process thus the output quality improvement by adopting the modified LTP methods, i.e., Eqs.

The solar wind and IMF data, and AL index shown in Fig. 4(c) represent that the geomagnetic activity and the solar wind were very quiet before the transient magnetopause entry.