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After transplantation, host flies were allowed to recover at room temperature for 1 2 h in fresh standard Drosophila medium before transferred to and maintained at 25 °C.
Each sample with equal amount of proteins (as the loading control) was separated on 12% SDS-PAGE before transferred onto a nitrocellulose membrane.
Once we got all set up, the harvester went to work, carefully harvesting one row at a time and then dumping the load in the wagon to be tested and weighed before transferred to the truck.
To facilitate DNA protein binding, we mixed the ds-oligo pool with 10 µg nuclear extract in 1× incubation buffer (Affymetrix, Santa Clara, CA, USA or Signosis, Santa Clara, CA, USA) at 15 oC for 30 min before transferred to the center of the Spin Columns.
All samples were stored in a refrigerator before transferred to other locations for analysis.
However, before transferred to the back-end calculating program, these inputs must be validated by the format authentication and the format validation programs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com