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After 1 week of recovery, these mice received daily subcutaneous injections with 100 μg/kg DES dissolved in olive oil or the olive oil vehicle alone for 1 week before they were terminated by cardiac puncture under isoflurane anesthesia.
dissolved in olive oil or the olive oil vehicle alone for 1 week before they were terminated by cardiac puncture under isoflurane anesthesia after which the uterus was weighed (n = 6 mice per group).
For the fourth experiment, 9-week-old male mice received daily subcutaneous injections with 100 μg/kg DES (Steraloids Inc., Newport, RI) dissolved in olive oil or the olive oil vehicle alone for 1 week before they were terminated by cardiac puncture under isoflurane anesthesia (n = 6 mice per group).
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Four telephone contact attempts for each household were made before attempts were terminated.
3H-thymidine was then added and cells were labeled for 6 hr before cultures were terminated and 3H-thymidine incorporation was assayed as previously described [ 19].
Agonists were then added for the times indicated (0.1 nM–3 µM, triplicate wells) before incubations were terminated by fixation with 3% paraformaldehyde in phosphate-buffered saline (PBS, 10 min at 21°C).
Figure 4 shows that the BC merges with PL 90 (i.e. the clusters were created before PL90 and were terminated after PL90).
Before her parental rights were terminated, Ana said, she completed a regimen of parenting courses and therapy sessions in hopes of being reunited with her family.
WorldCom is also effectively reducing the amount it will pay to 19 executives who were terminated before the company filed for bankruptcy.
The tech giant hopes to ax around 3,000 employees from its worldwide workforce, including 1,600 who were terminated before April 30.
Because the query disseminations in DRIVE were terminated before covering all of the selected road segments, its average query response time is much lower.
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CEO of Professional Science Editing for Scientists @ prosciediting.com