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Mice were then allowed to respond to the treatments for an additional 6 hr before they were euthanized.
The glycemia levels of the animals were checked once a week up to 24 h before they were euthanized.
None of the hamsters from the second passage of BSA-annealed fibrils developed clinical disease before they were euthanized at 661 days after inoculation.
The physical conditions of each animal were recorded before they were euthanized, according to the guidelines of the Japanese Veterinary Medical Association.
Trapped animals were placed in a plastic bag before they were euthanized with sodium pentobarbital (200 mg/kg; ethical approval reference number SU-ACUM11-00004 SU-ACUM11-00004 SU-ACUM11-00004
After this period, another group (n = 23) was euthanized and the remaining mice were fed the experimental diets (cholesterol (n = 25), formononetin (n = 23), or C7F (n = 25)) for additionally three weeks before they were euthanized.
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Explain that dogs and cats only have 3 days at the pound before they are euthanized or killed (in other words, by a shot).
From 3 days before the inoculation until they were euthanized, the animals were observed on a daily basis for clinical symptoms, and their rectal temperature was measured.
#Three monkeys from this group are not included in this calculation because they were euthanized before 45 months postinoculation for reasons unrelated to transmissible spongiform encephalopathy disease.
BrdU (50 mg kg−1 in saline) was administered intraperitoneally twice daily for 3 consecutive days before rats were euthanized.
Mice were randomly assigned into different groups (n=5) and grafts were left for 1, 3, 5, 7, 14 and 20 days before mice were euthanized by cervical dislocation.
More suggestions(15)
before they were booed
before they were scared
before they were baked
before they were collected
before they were deleted
before they were released
before they were removed
before they were elected
before they were tried
before they were invented
before they were asked
before they were called
before they were painted
before they were evicted
before they were hauled
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