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Hence, for longer silicon nanowires, the photon-generated carriers travel more distance before they were collected.
After 48 h, the cells were washed by PBS then trypsinized before they were collected into Falcon tubes for further analysis.
The cells were incubated for 1 h before they were collected, washed in PBS and lysed to determine the luciferase activity by using the dual luciferase reporter assay according to manufacturer's instructions (Promega), and read using a luminometer (Dynex).
All questionnaires were answered voluntarily and anonymized before they were collected.
In order to decrease the difference caused by culture nutrition, the cells were cultured for at least 24 hours in the same nutrition before they were collected.
Cells were seeded and incubated with Se-labeled selenite (Research Reactor Center, University of Missouri, Columbia, MO, USA) in a final concentration of 1 μCi/ml for 48 h before they were collected and lysed.
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In the MoS2/GaAs p-n junction, the recombination of photon-generated carriers can be enhanced due to the long transporting path in the thick MoS2 film before they are collected by the electrodes.
Over five years, the brines evaporate, concentrate, and travel several miles before they are collected as pure salt crystals.
Second, samples themselves may be contaminated with DNA of various sources, potentially even before they are collected [25], [26].
Even though our data about hospitalized patients and deaths were convenience samples, they were collected before the first reports were published suggesting that obesity may be associated with severe pandemic H1N1 disease.
Of the remaining 563 samples, 58 (10.3%) were rotavirus negative by RT-PCR and 56 were excluded because they were collected before April 2010; the latter group of samples comprised 15 G2P[6], 14 G12[8], 12 G2P[4], 3 G10P[8], 10 mixed infection, and 2 partially typed samples.
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before they were compiled
before they were connected
before they were completed
before they were consolidated
before they were selected
before they were analyzed
before they were examined
before they were reported
before they were washed
before they were used
before they were injected
before they were seen
before they were tested
before they were allowed
before they were placed
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