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The variability within individual animals should also be considered, the terminal study running several months before the time course study.
HeLa S3 were maintained between 5-10 × 105 cells/ml throughout the experiment and, before the time course, were grown in minimum essential Joklik modified media (Sigma Aldrich, St . Louis MO, USA) as previously described [ 22].
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gsyn (1000 ± 100 nS) and Vslope (20 ± 5 mV) were adjusted before experiment to match the time course of natural inhibition amplitude seen in VSI in that preparation.
Given that FAR is implicated in fatty acid metabolism, we next focused on fatty acid-related signaling in OE vs. Kan roots before and throughout the time course of inoculation.
The lag phase where [ATP]m falls before recovering, exactly matches the time course for [Ca2+]m to increase [64,76].
Time-dependent dissociation of the enzyme inhibitor complex produces a characteristic "lag" phase at the beginning of the time course before equilibration to a steady-state rate.
For electrical stimulation, pulses of 2 mA, 0.3 ms, and 2 Hz were applied 15 min before administration of LPS and during the time course of the experiment and were only interrupted during the neurophysiological measurements [11].
Before starting, we first checked that the time course of demyelination and remyelination in our transgenic mice was consistent with what is described for acute and chronic cuprizone models in the literature (supplementary material Fig. S1; for review, see Skripuletz et al., 2011).
So far, the time course of physiological data before performance have not been investigated within the probabilistic methodology framework.
However, it has also recently been found that another metabolic event occurs in response to LPS earlier in the time course of macrophage activation (before NO accumulation limits OXPHOS), which involves citrate.
Data for early adults derives from the 'before food removal' time point of the time course in Figure 3A.
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