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There were no a priori sample size calculations before the test sample.
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injected with PBS containing PO (200 mg/kg) 1 h before the test samples were administered to increase the serum uric acid level.
The cells were preincubated at 37°C for 15 minutes before stimulation with the test sample (diluted in 100 μl Ham's F-12 medium) for 5 minutes at 37°C.
Before starting the test, samples were dissolved in DMSO, at a concentration of 20 mg/mL, and at least five separate trials were made.
All animals were fasted for 15 h before administration of the test samples.
Before and after treatment, the test samples were ground and sieved into different fractions for physical analysis.
Before and after treatment, the test samples were ground and sieved into different fractions for chemical and physical analysis.
The pots were stored at greenhouse temperature conditions (23 ± 2 °C) and humidity of field capacity for 6 months before being used to prepare the test samples.
Control coverslips (no primary antibody) were checked for non-specific binding before assessing staining intensity in the test samples.
Rehydration of the test samples before applying hydrogen peroxidase to the test windows increases sensitivity, but is not recommended [ 9] because it increases the false positive rate, thus reducing the positive predictive value by more than 50% [ 11].
Before the spiking and recovery study, each test sample was verified for no parathion-methyl, parathion, and fenitrothion in the tested samples by gas chromatography (limit of quantification was 40 ng g 1).
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