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When gfp was inserted into the fosmid precisely after the initiation codon or before the termination codon (fUL HF001.1 and fUL HF002.1, respectively) so as to encode N-terminal and C-terminal CEH-63 translational fusions, expression was again observed in DVC and the uterus (Figs. 3D to I).
The length of the longest ORF was defined as the distance between the nucleotide position (number) of the A in ATG and the last nucleotide before the termination codon.
As annotated, there are two amino acids before the termination codon.
Reporter genes were inserted immediately after the initiation codon in the starting exon of each transcript and also before the termination codon common to all transcripts.
Nevertheless, insertion of gfp after either initiation codon or before the termination codon yielded reporter expression and with closely related patterns (WBIDs Expr9810/9814).
The reverse primer starts from the codon immediately before the termination codon and carried the Gateway B2.1 sequence at its 5' end.
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In the other two frames there would be 16 amino acids or no amino acids before the termination codons in those frames (Table 2).
Mutation of the termination codon resulted in production of a C-terminal elongated protein.
The resulting exoinzed transcripts were divided into 5 types by location of the termination codon (PTC) (Figure 1).
The first ATG codon of the sequence at position 50-52 wassignedned as the translational initiation codon and the TAA codon at position 611-613 as the termination codon.
In strains YJM789, YJM456, YJM969, and DTY3, the termination codon of CUP1 was UAA instead of UGA, the termination codon for CUP1 in S288c and the other strains.
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