Sentence examples for before the stop codon from inspiring English sources

A His-tag sequence was also inserted at the 3′ region of the apoptin gene before the stop codon (data not shown and Fig. 1).

Here we engineered the previously described HEL24.3 hiPSC to generate a SOX2 reporter by knocking-in a T2A fused nuclear tdTomato reporter cassette before the STOP codon of the SOX2 gene coding sequence.

The 10 nucleotide insertion found in patients with FDD was introduced before the stop codon.

The ORF-specific region of the forward primers began with the start codon and the reverse primers began with the final nucleotide before the stop codon.

For the HML-HIS4 interval, we used the S. cerevisiae sequences outside of HML to the nucleotide before the stop codon of HIS4 (chromosomal co-ordinates 14850 65933).

The reverse ORF-specific primers begin with the nucleotide before the stop codon in order to allow C-terminal fusion proteins to be expressed from destination vectors.

The coding region of human ENGASE was amplified by PCR: the his6-tag sequence was added in-frame immediately before the stop codon.

Duf flag was generated by inserting the Flag-epitoe before the stop codon at the C terminus of Duf in pCI-neo and subcloned into pAC5.1A and pUAST.

After the entire coding region was subcloned into pBluescriptII SK−, DNA sequences encoding three consecutive repeats of the FLAG tag (DYKDDDDK) or HA tag were inserted just before the stop codon.

For the entire coding region of the yata gene, DNA sequences encoding one lysine and three consecutive repeats of the HA tag were inserted just before the stop codon.

Stop codons located in the last exon do not induce NMD, probably because the translating ribosome displaces the EJC before reaching the stop codon.