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Before the statistical analyses of the fast component of the activation for Kv7.2 and Kv7.2-W236L the measured time constants were log-transformed to meet the assumption of normality.
The quality of data was checked with good correlation (Additional file 2: Figure S1) before the statistical analyses.
An underlying assumption about the significance level is that the estimate is developed from a model that was specified before the statistical analyses were performed.
We therefore carefully excluded blood samples that might have been strongly influenced by other sources than air pollution before the statistical analyses.
To limit the number of independent variables and simplify the analyses, these two measures were therefore averaged into one xy-position variable before the statistical analyses.
Insulin, glucagon, glucose, lactate, and NEFAs were log transformed before the statistical analyses to favor normality of residuals and homogeneity of variance.
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This was evaluated by the Kolmogorov-Smirnoc test before performing the statistical analyses.
Before conducting the statistical analyses, we examined the data for univariate and multivariate outliers.
All the patient data were anonymized after collecting the clinicopathological variables for each patient and before doing the statistical analyses.
All patient data were anonymized after collecting the clinicopathological variables for each patient and before doing the statistical analyses.
Appropriate transformations will be made on skewed variables to correct their skewness before being entered into the statistical analyses.
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